Our scientific research

In cooperation with University of Wageningen and veterinarian Annelies van Tilburg (she passed away 26-1-2014) we have designed and carried out a most solid RCT studie on 47 dogs, suffering from atopic- and constitutional allergies and other Non-Systemical manifestations; randomized, double-blind, split-sample and retropespective*). Our study did run until December 2013 and is published in August 2014.

See our publication on an Open Access website, to enable everybody to read our publication and/or to download our publication freely  at  scientific magazine: 'International Journal of Biology, Veterinary, Agricultural and Food Engineering' Vol:8 no: 7, 2014  Publication number 189 



Abstract:

Background

Food-related allergies and intolerances are frequently occurring in dogs. Diagnosis and monitoring according ‘Golden Standard’ of elimination efficiency is, however, time consuming, expensive, and requires expert clinical setting. In order to facilitate rapid and robust, quantitative testing of intolerance, and determining the individual offending foods, a serological test is implicated for Alimentary Induced Diseases and manifestations.

Method
As we developed Medisynx IgG Human Screening Test ELISA before and the dog’ immune system is most similar to humans, we were able to develop Medisynx IgG Dog Screening Test ELISA as well.

In this randomized, double-blind, split-sample, retro perspective study 47 dogs suffering from Canine Atopic Dermatitis (CAD) and several secondary induced reactions were included to participate in serological Medisynx IgG Dog Screening Test ELISA (within < 0,02 % SD).

Results were expressed as titers relative to the standard OD readings to diagnose alimentary induced diseases and monitoring efficacy of an individual eliminating diet in dogs. Split sample analysis was performed by independently sending 2 times 3 ml serum under two unique codes.

Results
The veterinarian monitored these dogs to check dog’ results at least at 3, 7, 21, 49, 70 days and after period of 6 and 12 months on an individual negative diet and a positive challenge (retrospectively) at 6 months. Data of each dog were recorded in a screening form and reported that a complete recovery of all clinical manifestations was observed at or less than 70 days (between 50 and 70 days) in the majority of dogs (44 out of 47 dogs = 93.6%). Conclusion. Challenge results showed a significant result of 100% in specificity as well as 100% positive predicted value. On the other hand, sensitivity was 95,7% and negative predictive value was 95,7%.

Conclusion

In conclusion, an individual diet based on IgG ELISA in dogs provides a significant improvement of atopic dermatitis and pruritus including all other non-specific defined allergic skin reactions as erythema, itching, biting and gnawing at toes, as well as to several secondary manifestations like chronic diarrhoea, chronic constipation, otitis media, obesity, laziness or inactive behaviour, pain and muscular stiffness causing a movement disorders, excessive lacrimation, hyper behaviour, nervous behaviour and not possible to stay alone at home, anxiety, biting and aggressive behaviour and disobedience behaviour. Furthermore, we conclude that a relatively more severe systemic candidiasis, as shown by relatively higher titer (class 3 and 4 IgG reactions to Candida albicans), influence the duration of recovery from clinical manifestations in affected dogs. These findings are consistent with our preliminary human clinical studies.



Our total scientific team worked on basis of Non-Profit, so without any salary in our own free time. We are very proud on our team.


*) explanation of terms:

Randomized, means we have selected these dogs at random and devided them at random into groups.

Double- blind, means all members of our scientific team had only insight in their own part of the study and were not able to obtain details or results of the rest of study. After closing the study we open the data to analyze and to conclude.

Split-sample, means that independant veterinarians took of blood sample and devided it into two separate samples send in separtely by an unique code to Medisynx laboratory and were perfomed separately. This is a scientific way to prove that we are
able to reproduce any of our test twice with exact the same outcomes and this is a must in science.

Retroperspective, means that we carried out a final challange and the end after we were able to show which dog got wrong or right diet at any moment, and that we can repeat it.





 

 

Our scientific research

In cooperation with University of Wageningen and veterinarian Annelies van Tilburg (she passed away 26-1-2014) we have designed and carried out a most solid RCT studie on 47 dogs, suffering from atopic- and constitutional allergies and other Non-Systemical manifestations; randomized, double-blind, split-sample and retropespective*). Our study did run until December 2013 and is published in August 2014.

See our publication on an Open Access website, to enable everybody to read our publication and/or to download our publication freely  at  scientific magazine: 'International Journal of Biology, Veterinary, Agricultural and Food Engineering' Vol:8 no: 7, 2014  Publication number 189 



Abstract:

Background

Food-related allergies and intolerances are frequently occurring in dogs. Diagnosis and monitoring according ‘Golden Standard’ of elimination efficiency is, however, time consuming, expensive, and requires expert clinical setting. In order to facilitate rapid and robust, quantitative testing of intolerance, and determining the individual offending foods, a serological test is implicated for Alimentary Induced Diseases and manifestations.

Method
As we developed Medisynx IgG Human Screening Test ELISA before and the dog’ immune system is most similar to humans, we were able to develop Medisynx IgG Dog Screening Test ELISA as well.

In this randomized, double-blind, split-sample, retro perspective study 47 dogs suffering from Canine Atopic Dermatitis (CAD) and several secondary induced reactions were included to participate in serological Medisynx IgG Dog Screening Test ELISA (within < 0,02 % SD).

Results were expressed as titers relative to the standard OD readings to diagnose alimentary induced diseases and monitoring efficacy of an individual eliminating diet in dogs. Split sample analysis was performed by independently sending 2 times 3 ml serum under two unique codes.

Results
The veterinarian monitored these dogs to check dog’ results at least at 3, 7, 21, 49, 70 days and after period of 6 and 12 months on an individual negative diet and a positive challenge (retrospectively) at 6 months. Data of each dog were recorded in a screening form and reported that a complete recovery of all clinical manifestations was observed at or less than 70 days (between 50 and 70 days) in the majority of dogs (44 out of 47 dogs = 93.6%). Conclusion. Challenge results showed a significant result of 100% in specificity as well as 100% positive predicted value. On the other hand, sensitivity was 95,7% and negative predictive value was 95,7%.

Conclusion

In conclusion, an individual diet based on IgG ELISA in dogs provides a significant improvement of atopic dermatitis and pruritus including all other non-specific defined allergic skin reactions as erythema, itching, biting and gnawing at toes, as well as to several secondary manifestations like chronic diarrhoea, chronic constipation, otitis media, obesity, laziness or inactive behaviour, pain and muscular stiffness causing a movement disorders, excessive lacrimation, hyper behaviour, nervous behaviour and not possible to stay alone at home, anxiety, biting and aggressive behaviour and disobedience behaviour. Furthermore, we conclude that a relatively more severe systemic candidiasis, as shown by relatively higher titer (class 3 and 4 IgG reactions to Candida albicans), influence the duration of recovery from clinical manifestations in affected dogs. These findings are consistent with our preliminary human clinical studies.



Our total scientific team worked on basis of Non-Profit, so without any salary in our own free time. We are very proud on our team.


*) explanation of terms:

Randomized, means we have selected these dogs at random and devided them at random into groups.

Double- blind, means all members of our scientific team had only insight in their own part of the study and were not able to obtain details or results of the rest of study. After closing the study we open the data to analyze and to conclude.

Split-sample, means that independant veterinarians took of blood sample and devided it into two separate samples send in separtely by an unique code to Medisynx laboratory and were perfomed separately. This is a scientific way to prove that we are
able to reproduce any of our test twice with exact the same outcomes and this is a must in science.

Retroperspective, means that we carried out a final challange and the end after we were able to show which dog got wrong or right diet at any moment, and that we can repeat it.